ChloroquineV1, Main, Exploration, bibRecord, 000857

Effect of silencing S-phase kinase-associated protein 2 on chemosensitivity to temozolomide of human glioma cells U251

Identifieur interne : 000857 ( Main/Exploration ); précédent : 000856; suivant : 000858

Effect of silencing S-phase kinase-associated protein 2 on chemosensitivity to temozolomide of human glioma cells U251

Auteurs : Yue Liu ; Zhen-Gang Tang ; Jian-Quan Yang ; Yi Zhou ; Yi Lin ; Wei Lv ; Guo-Bin Wang ; Cai-Li Li

Source :

American Journal of Translational Research [ 1943-8141 ] ; 2019.

RBID : PMC:6511802

Abstract

Objective: To examine the effect of silencing SKP2 on chemosensitivity of human glioma cells U251 to temozolomide (TMZ). Methods: Adenoviruses harbouring shRNA targeting SKP2 (i.e. Ad-shSKP2) and non-targeting scrambled shRNA (i.e. Ad-shNC) were used to infect U251 cells. The transduced cells were then treated with TMZ. Cell viability after treatment was assayed using CCK8; while cell cycle and apoptosis were examined using flow cytometry. To study the effect of silencing SKP2 on autophagy in U251, we co-transduced the cells with Ad-mRFP-LC3 and Ad-shSKP2/Ad-shNC. The expression of autophagy marker LC3 after TMZ treatment was studied using microscopy and Western blotting assays. Results: The cytotoxicity of TMZ (i.e. 20-100 µM) was more significantly seen in Ad-shSKP2-transduced U251 cells than in the Ad-shNC-transduced U251 cells. The IC₅₀ values in shSKP2-U251 were significantly lower than those of the shNC-U251 (P < 0.05). Both TMZ and Ad-shSKP2 alone increased apoptosis and promoted expression of LC3 in U251. Combined treatment of Ad-shSKP2 and TMZ further elevated apoptosis and LC3 expression. Conclusion: Silencing SKP2 in U251 cells increased chemosensitivity to TMZ that was accompanied with enhanced apoptosis and autophagy. Targeting SKP2 may be a potential approach to potentiate TMZ treatment in patients with glioma.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511802

PubMed: 31105854
PubMed Central: 6511802

Affiliations:

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<author><name sortKey="Yang, Jian Quan" sort="Yang, Jian Quan" uniqKey="Yang J" first="Jian-Quan" last="Yang">Jian-Quan Yang</name>
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<author><name sortKey="Zhou, Yi" sort="Zhou, Yi" uniqKey="Zhou Y" first="Yi" last="Zhou">Yi Zhou</name>
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<front><div type="abstract" xml:lang="en"><p>Objective: To examine the effect of silencing SKP2 on chemosensitivity of human glioma cells U251 to temozolomide (TMZ). Methods: Adenoviruses harbouring shRNA targeting SKP2 (i.e. Ad-shSKP2) and non-targeting scrambled shRNA (i.e. Ad-shNC) were used to infect U251 cells. The transduced cells were then treated with TMZ. Cell viability after treatment was assayed using CCK8; while cell cycle and apoptosis were examined using flow cytometry. To study the effect of silencing SKP2 on autophagy in U251, we co-transduced the cells with Ad-mRFP-LC3 and Ad-shSKP2/Ad-shNC. The expression of autophagy marker LC3 after TMZ treatment was studied using microscopy and Western blotting assays. Results: The cytotoxicity of TMZ (i.e. 20-100 µM) was more significantly seen in Ad-shSKP2-transduced U251 cells than in the Ad-shNC-transduced U251 cells. The IC<sub>50</sub>
 values in shSKP2-U251 were significantly lower than those of the shNC-U251 (<italic>P</italic>
 < 0.05). Both TMZ and Ad-shSKP2 alone increased apoptosis and promoted expression of LC3 in U251. Combined treatment of Ad-shSKP2 and TMZ further elevated apoptosis and LC3 expression. Conclusion: Silencing SKP2 in U251 cells increased chemosensitivity to TMZ that was accompanied with enhanced apoptosis and autophagy. Targeting SKP2 may be a potential approach to potentiate TMZ treatment in patients with glioma.</p>
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<name sortKey="Liu, Yue" sort="Liu, Yue" uniqKey="Liu Y" first="Yue" last="Liu">Yue Liu</name>
<name sortKey="Lv, Wei" sort="Lv, Wei" uniqKey="Lv W" first="Wei" last="Lv">Wei Lv</name>
<name sortKey="Tang, Zhen Gang" sort="Tang, Zhen Gang" uniqKey="Tang Z" first="Zhen-Gang" last="Tang">Zhen-Gang Tang</name>
<name sortKey="Wang, Guo Bin" sort="Wang, Guo Bin" uniqKey="Wang G" first="Guo-Bin" last="Wang">Guo-Bin Wang</name>
<name sortKey="Yang, Jian Quan" sort="Yang, Jian Quan" uniqKey="Yang J" first="Jian-Quan" last="Yang">Jian-Quan Yang</name>
<name sortKey="Zhou, Yi" sort="Zhou, Yi" uniqKey="Zhou Y" first="Yi" last="Zhou">Yi Zhou</name>
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Effect of silencing S-phase kinase-associated protein 2 on chemosensitivity to temozolomide of human glioma cells U251

Effect of silencing S-phase kinase-associated protein 2 on chemosensitivity to temozolomide of human glioma cells U251

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Abstract

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